OECD 471: Bacterial reverse mutation test (Ames)
When bacterial strains are exposed to a mutagen, some of the bacteria in the treated population undergo genetic changes that cause them to revert to a prototrophic state and thus grow in the absence of exogenous amino acids.
Different tester strains have different sensitivities and responses to known mutagens; therefore, using a range of tester strains increases the sensitivity of the assay to detect any mutagenic activity.
- Plate incorporation (OECD 471 method for treating and plating)
- Urine mutagenicity
- 6-well and 24-well Ames II screen