Gene Expression Analysis

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Gene expression profiling analysis is an important part of the drug development process. It is a powerful tool that can be used to uncover the complex molecular "signature" of a disease and provide information that can determine efficacy of new therapeutic agents.

Harnessing the power of NanoString and other PCR-based technologies, Labcorp can provide a variety of gene expression analysis services (DNA and RNA) to allow researchers to gain a molecular snapshot of the tumor microenvironment and to provide answers that can assist researchers with decisions during early-stage drug discovery by confirming the expected mechanism of action and target engagement of a therapeutic. These services may also be applied to cell culture studies for proof-of-concept research prior to advancing to in vivo models.

  • NanoString

    A recent addition to Labcorp’s service portfolio, NanoString’s nCounter® Analysis System and the GeoMx® Digital Spatial Profiler (DSP) can be used to inform how a drug modulates gene expression and the signaling pathways that regulate tumor growth.

    The NanoString nCounter® allows for analysis of up to 800 RNA, DNA or protein targets simultaneously with high sensitivity and precision. The system utilizes molecular "barcodes" and single molecule imaging to quantitate hundreds of unique transcripts in a single sample.

    Other key advantages of using the NanoString nCounter® include:

    • A number of “off-the-shelf panels” for oncology (human and mouse) available, customizable to include up to 55 additional unique targets
    • Optimized for challenging sample types (FFPE, tissue, lysates and biofluids)
    • Only low amounts of precious sample material required
    • Accurate and faster than qPCR (no amplification requirements) and simpler than next generation sequencing

    While NanoString nCounter® provides great quantitative data on gene or protein expression levels from tumor samples, spatial context is key to gaining further insights into tissue biology. NanoString GeoMx® DSP is the spatial biology solution and it can be used to gain a deeper understanding of the tumor and the complex interactions within the tumor microenvironment.

    The NanoString GeoMx® DSP technology builds upon the highly robust NanoString nCounter® digital barcode technology, providing morphological context and high-plex protein or gene expression profiling from just one prepared slide sample. The technology uses software that offers tremendous flexibility when selecting a region-of-interest strategy (geometric, segmentation, cell-type specific, contour, gridded) to visualize spatial architecture and analyze multiple biomarkers of a tissue sample. The data analysis software in GeoMx® DSP supports easy visualization and interpretation of data. High-resolution images can be displayed alongside quantitative profiling data, allowing interaction with either dataset in real time.

    Labcorp is the only preclinical CRO, globally, to offer NanoString’s GeoMx® DSP technology.

    In addition to being utilized as a standalone in vitro service for genetic expression screening using cell-based models, NanoString may also be used in an ex vivo setting to analyze the drug mechanism of action in in vivo animal models, thereby offering the ability to screen multiple therapeutics to identify promising lead compounds.

  • PCR en tiempo real

    Usamos un sistema PCR en tiempo real StepOnePlus™ que puede leer cuatro colores diferentes en una placa de 96 pocillos y se puede usar en ensayos como genotipificación de SNP, análisis de expresión génica, expresión de microARN, análisis de translocación y detección génica. Nuestros especialistas en PCR le ayudan a determinar la expresión de genes específicos en células cultivadas, tejidos frescos o tejidos congelados. Si necesita medir la expresión de genes en diferentes líneas celulares, cepas de ratones, tejidos o tumores, o monitorear la expresión de diferentes genes con diferentes tratamientos, nuestros especialistas en PCR pueden ayudarle a diseñar un estudio que se adapte a sus necesidades.

  • Perfilado de expresión génica en líneas celulares y tumores

    El perfilado de expresión génica es la medición de la actividad (la expresión) de miles de genes a la vez para crear una imagen global de la función celular. El ARN se secuencia usando secuenciamiento de nueva generación, se cuantifica y se alínea con miles de secuencias de ARN conocidas de genes específicos. Estos perfiles pueden, por ejemplo, distinguir entre células que se están dividiendo activamente, mostrar cómo las células reaccionan a un tratamiento particular o determinar qué genes se expresan diferencialmente en diferentes líneas celulares, cepas de ratones, tejidos o tumores. Labcorp collaborates with a partner laboratory that specializes in sequencing the RNA. Una vez que obtenemos la información de secuenciamiento, tenemos el software para cuantificar, alinear y analizar los datos. Podemos comparar la expresión de genes individuales, conjuntos de genes relacionados, grupos de genes específicos de una vía o un proceso, y genomas enteros entre muestras y mostrar los resultados comparativos en gráficos y cifras puntuales.

Access a customized offering to meet your gene expression requirements

At Labcorp, we combine state-of-the-art technologies with a customized service offering to meet your gene expression requirements. Our highly specialized team has a wealth of knowledge and experience in developing, optimizing, and validating gene expression readouts throughout the entire drug discovery process. We work closely with you to design a tailored workflow to generate a wealth of data to help you uncover the mechanism of action of your compound(s) and what signaling pathways and/or downstream genes are regulated in the context of tumor growth. We pride ourselves in having an extensive portfolio of animal/tumor models to ensure we can provide our clients with the “best fit” model for their project. Contact us today to discuss your study requirements and how our gene expression analysis services can add new insights into your research projects. 

Spotlight

El panel nCounter® IO 360™ y citometría de flujo combinan perfiles inmunofenotípicos de subconjuntos inmunológicos y transcriptomas para ofrecer un análisis integral de una inmunoterapia tumoral

Transcriptomic profiling can be used to examine the interaction between the tumor microenvironment (TME) and the immune system and is a powerful tool to investigate the mechanistic action of immunotherapies. In this Tech Spotlight, we demonstrate how gene expression measured in solid tumors using the nCounter® PanCancer IO 360™ panel (NanoString Technologies) can be combined with flow cytometry to provide a comprehensive evaluation of the effects that immunotherapy has on the anti-tumor immune response.

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